protective effects of the morus alba l. leaf extracts on cisplatin-induced nephrotoxicity in rat

نویسندگان

m nematbakhsh

v hajhashemi

a ghannadi

a talebi

چکیده

cisplatin (cp) as an important anti-tumor drug causes nephrotoxicity mainly by oxidative stress and renin-angiotensin system (ras). since flavonoids have high antioxidant activity and probable role in the inhibition of ras, this study was designed to investigate the protective effect of hydroalcoholic extract and flavonoid fraction of morus alba leaves on cisplatin-induced nephrotoxicity in rat. extracts of morus alba leaves were prepared and analyzed phytochemically. male rats (160-200 g) were used in this study (n=7-9). normal group received 0.2 ml normal saline intraperitoneally (i.p.) once daily for ten days. control animals received cp on the third day and saline in the remaining days. other groups received either hydroalcoholic extract (200, 400 and 600 mg/kg, i.p.) or flavonoid fraction (50, 100 and 200 mg/kg, i.p.) for two days before cp administration and thereafter until tenth day. serum concentrations of blood urea nitrogen (bun), creatinine (cr) and nitric oxide were measured using standard methods. also left kidneys were prepared for pathological study. the serum levels of bun and cr increased in animals received cp. hydroalcoholic extract was ineffective in reversing these alterations but flavonoid fraction (50 and 100 mg/kg) significantly inhibited cp-induced increases of bun and cr. none of the treatments could affect serum concentration of nitric oxide. flavonoid fraction could also prevent cp-induced pathological damage of the kidney. it seems that concurrent use of flavonoid fraction of morus alba with cp can protect kidneys from cp-induced nephrotoxicity.

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Protective effects of the Morus alba L. leaf extracts on cisplatin-induced nephrotoxicity in rat

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عنوان ژورنال:
research in pharmaceutical sciences

جلد ۸، شماره ۲، صفحات ۷۱-۰

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